Journal: ACS Applied Materials & Interfaces

Article Title: Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes

doi: 10.1021/acsami.0c08033

Figure Lengend Snippet: Cellular localization of Ru–PhenAN in live HeLa cells. HeLa cells were incubated with 30 μg/mL Ru–PhenAN in complete medium for 1 h. Then, live cells were imaged every 30 s for a total of 20 min on a DeltaVision Elite fluorescence wide field microscope. (a) Images show that the nuclear signal of Ru–PhenAN increases over time. Top: merged bright-field image and Ru–PhenAN fluorescence signal (red). Bottom: Ru–PhenAN fluorescence signal only (white). Scale bar: 25 μm. The corresponding Video S1 is included in the Supporting Information. (b) Fluorescence intensity (raw integrated density) in eight arbitrary selections of the same size taken from the image shown on the left. Left image: the areas selected for the calculation of the fluorescence intensities are indicated with yellow circles. Right graph: fluorescence intensity over time of the areas selected. When the nuclei were selected (black symbols, nuclei), an increase in signal over time was measured. When regions outside the nuclei were selected (red symbols, background), the signal remained constant over time. This quantification confirmed an increase in Ru–PhenAN fluorescence inside the nuclei upon illumination, as observed in panel a and the corresponding Supporting Video S1 .

Article Snippet: HeLa cells were seeded at a density of 100,000 cells per microscope dish (35 mm glass bottom dishes, MatTek) and incubated at 37 °C in 5% CO 2 for 24 h. Then, cells were exposed to 1 mL of Ru–PhenAN at the required concentration in complete medium.

Techniques: Incubation, Fluorescence, Microscopy

Journal: ACS Applied Materials & Interfaces

Article Title: Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes

doi: 10.1021/acsami.0c08033

Figure Lengend Snippet: Cellular localization of Ru–PhenAN in live HeLa cells depends on the sample illumination. Cells were exposed to 30 μg/mL Ru–PhenAN in complete medium for 24 h in the dark and then imaged on a Leica SP8 confocal microscope, opening the pinhole size to 2.0 airy units to increase the recorded signal. Before acquiring the image, the sample area delimited by the yellow line was illuminated every 5 s for 10 min using the microscope laser, while the area within the blue line was not illuminated. Only in the illuminated area was the Ru–PhenAN signal at the nuclear level observed, whereas it remained confined in vesicles in the non-illuminated area. Scale bar: 50 μm.

Article Snippet: HeLa cells were seeded at a density of 100,000 cells per microscope dish (35 mm glass bottom dishes, MatTek) and incubated at 37 °C in 5% CO 2 for 24 h. Then, cells were exposed to 1 mL of Ru–PhenAN at the required concentration in complete medium.

Techniques: Microscopy